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Publications

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  • Hawes EM, Rahim M, Haratipour Z, Orun AR, O’Rourke ML, Oeser JK, Kim K, Claxton DP, Blind RD, Young JD, O’Brien RM. Biochemical and metabolic characterization of a G6PC2 inhibitor. Biochimie, 222, 109, 2024. https://doi.org/10.1016/j.biochi.2024.02.012

  • Sinclair, M., Stein, R. A., Sheehan, J. H., Hawes, E. M., O’Brien, R. M., Tajkhorshid, E., & Claxton, D. P. (2024). Integrative analysis of pathogenic variants in glucose-6-phosphatase based on an AlphaFold2 model. PNAS Nexus, 3(2), pgae036. https://doi.org/10.1093/pnasnexus/pgae036

  • Hawes, E. M., Claxton, D. P., Oeser, J. K., & O’Brien, R. M. (2023). Identification of structural motifs critical for human G6PC2 function informed by sequence analysis and an AlphaFold2-predicted model. Bioscience Reports, 44(1). https://doi.org/10.1042/bsr20231851

  • Overway, E. M., Bosma, K. J., Claxton, D. P., Oeser, J. K., Singh, K., Breidenbach, L. B., … O’Brien, R. M. (2022a). Nonsynonymous single-nucleotide polymorphisms in the G6PC2 gene affect protein expression, enzyme activity, and fasting blood glucose. Journal of Biological Chemistry, 298(2), 101534. doi:10.1016/j.jbc.2021.101534

  • Claxton, D. P., Overway, E. M., Oeser, J. K., O’Brien, R. M., & Mchaourab, H. S. (2022). Biophysical and functional properties of purified glucose-6-phosphatase catalytic subunit 1. Journal of Biological Chemistry, 298(1), 101520. doi:10.1016/j.jbc.2021.101520

  • Claxton, D. P., Jagessar, K. L., & Mchaourab, H. S. (2021). Principles of alternating access in multidrug and toxin extrusion (mate) transporters. Journal of Molecular Biology, 433(16), 166959. doi:10.1016/j.jmb.2021.166959

  • Castellano, S., Claxton, D. P., Ficici, E., Kusakizako, T., Stix, R., Zhou, W., … Faraldo-Gómez, J. D. (2021). Conserved binding site in the N-Lobe of Prokaryotic Mate Transporters suggests a role for na+ in ion-coupled drug efflux. Journal of Biological Chemistry, 296, 100262. doi:10.1016/j.jbc.2021.100262

  • Han, B., Porta, J. C., Hanks, J. L., Peskova, Y., Binshtein, E., Dryden, K., Claxton, D. P., Mchaourab, H. S., Karakas, E., Ohi, M. D., & Kenworthy, A. K. (2020). Structure and assembly of CAV1 8S complexes revealed by single particle electron microscopy. Science Advances, 6(49). https://doi.org/10.1126/sciadv.abc6185

  • Jagessar, K. L., Claxton, D. P., Stein, R. A., & Mchaourab, H. S. (2020). Sequence and structural determinants of ligand-dependent alternating access of a MATE transporter. Proceedings of the National Academy of Sciences, 117(9), 4732–4740. https://doi.org/10.1073/pnas.1917139117

  • Jagessar, K. L., Mchaourab, H. S., & Claxton, D. P. (2019). The N-terminal domain of an archaeal multidrug and toxin extrusion (MATE) transporter mediates proton coupling required for prokaryotic drug resistance. Journal of Biological Chemistry, 294(34), 12807–12814. https://doi.org/10.1074/jbc.ra119.009195

  • Kusakizako, T., Claxton, D. P., Tanaka, Y., Maturana, A. D., Kuroda, T., Ishitani, R., Mchaourab, H. S., & Nureki, O. (2018). Structural basis of H+-Dependent conformational change in a bacterial MATE transporter. Structure, 27(2), 293-301.e3. https://doi.org/10.1016/j.str.2018.10.004

  • Mishra, S., Chandler, S. A., Williams, D., Claxton, D. P., Koteiche, H. A., Stewart, P. L., Benesch, J. L., & Mchaourab, H. S. (2018). Engineering of a polydisperse small Heat-Shock protein reveals conserved motifs of oligomer plasticity. Structure, 26(8), 1116-1126.e4. https://doi.org/10.1016/j.str.2018.05.015

  • Phulera, S., Zhu, H., Yu, J., Claxton, D. P., Yoder, N., Yoshioka, C., & Gouaux, E. (2018). Cryo-EM structure of the benzodiazepine-sensitive α1β1γ2S tri-heteromeric GABAA receptor in complex with GABA. eLife, 7. https://doi.org/10.7554/elife.39383

  • Claxton, D. P., Jagessar, K. L., Steed, P. R., Stein, R. A., & Mchaourab, H. S. (2018). Sodium and proton coupling in the conformational cycle of a MATE antiporter from Vibrio cholerae. Proceedings of the National Academy of Sciences, 115(27), E6182–E6190. https://doi.org/10.1073/pnas.1802417115

  • Paz, A., Claxton, D. P., Kumar, J. P., Kazmier, K., Bisignano, P., Sharma, S., Nolte, S. A., Liwag, T. M., Nayak, V., Wright, E. M., Grabe, M., Mchaourab, H. S., & Abramson, J. (2018). Conformational transitions of the sodium-dependent sugar transporter, vSGLT. Proceedings of the National Academy of Sciences, 115(12), E2742–E2751. https://doi.org/10.1073/pnas.1718451115

  • Kazmier, K., Claxton, D. P., & Mchaourab, H. S. (2016). Alternating access mechanisms of LeuT-fold transporters: trailblazing towards the promised energy landscapes. Current Opinion in Structural Biology, 45, 100–108. https://doi.org/10.1016/j.sbi.2016.12.006

  • Koteiche, H. A., Claxton, D. P., Mishra, S., Stein, R. A., McDonald, E. T., & Mchaourab, H. S. (2015). Species-Specific structural and functional divergence of Α-Crystallins: zebrafish ΑBA- and rodent ΑAins-Crystallin encode activated chaperones. Biochemistry, 54(38), 5949–5958. https://doi.org/10.1021/acs.biochem.5b00678

  • Claxton, D. P., Kazmier, K., Mishra, S., & Mchaourab, H. S. (2015). Navigating membrane protein structure, dynamics, and energy landscapes using spin labeling and EPR spectroscopy. Methods in Enzymology on CD-ROM/Methods in Enzymology, 564, 349–387. https://doi.org/10.1016/bs.mie.2015.07.026

  • Goehring, A., Lee, C., Wang, K. H., Michel, J. C., Claxton, D. P., Baconguis, I., Althoff, T., Fischer, S., Garcia, K. C., & Gouaux, E. (2014). Screening and large-scale expression of membrane proteins in mammalian cells for structural studies. Nature Protocols, 9(11), 2574–2585. https://doi.org/10.1038/nprot.2014.173

  • Claxton, D. P., Quick, M., Shi, L., De Carvalho, F. D., Weinstein, H., Javitch, J. A., & Mchaourab, H. S. (2010). Ion/substrate-dependent conformational dynamics of a bacterial homolog of neurotransmitter:sodium symporters. Nature Structural & Molecular Biology, 17(7), 822–829. https://doi.org/10.1038/nsmb.1854

  • Kumar, M. S., Koteiche, H. A., Claxton, D. P., & Mchaourab, H. S. (2008). Disulfide cross‐links in the interaction of a cataract‐linked αA‐crystallin mutant with βB1‐crystallin. FEBS Letters, 583(1), 175–179. https://doi.org/10.1016/j.febslet.2008.11.047

  • Claxton, D. P., Zou, P., & Mchaourab, H. S. (2007). Structure and orientation of T4 lysozyme bound to the small heat shock protein Α-Crystallin. Journal of Molecular Biology, 375(4), 1026–1039. https://doi.org/10.1016/j.jmb.2007.11.014

  • Gavira, J. A., Claxton, D. P., Wang, J. H., Toh, D., Meehan, E. J., & DiGiammarino, E. L. (2003). Purification, crystallization and preliminary X-ray analysis ofCaenorhabditis elegansubiquitin-conjugation enzyme M7.1. Acta Crystallographica Section D Biological Crystallography, 59(3), 544–546. https://doi.org/10.1107/s0907444903000039