Skip to main content

Proteomics characterization of exosome cargo.


AUTHORS

Schey| Luther| Rose KL| JM| KLKevin L| J Matthew| Kristie L . Methods (San Diego, Calif.). 2015 10 1; 87(). 75-82

ABSTRACT

Characterization of exosomal cargo is of significant interest because this cargo can provide clues to exosome biogenesis, targeting, and cellular effects and may be a source of biomarkers for disease diagnosis, prognosis and response to treatment. With recent improvements in proteomics technologies, both qualitative and quantitative characterization of exosomal proteins is possible. Here we provide a brief review of exosome proteomics studies and provide detailed protocols for global qualitative, global quantitative, and targeted quantitative analysis of exosomal proteins. In addition, we provide an example application of a standard global quantitative analysis followed by validation via a targeted quantitative analysis of urine exosome samples from human patients. Advantages and limitations of each method are discussed as well as future directions for exosome proteomics analysis.



Leave a Response


Warning: Undefined variable $user_ID in /var/www/m_lab.dev.vanderbilt.edu/wp-content/themes/ANCHORDOWN-Vanderbilt/comments.php on line 62