2024
Islam M,
Yang Y,
Simmons AJ,
Shah VM,
Musale KP,
Xu Y,
Tasneem N,
Chen Z,
Trinh LT,
Molina P,
Ramirez-Solano MA,
Sadien ID,
Dou J,
Rolong A,
Chen K,
Magnuson MA,
Rathmell JC,
Macara IG,
Winton DJ,
Liu Q,
Zafar H,
Kalhor R,
Church GM,
Shrubsole MJ,
Coffey RJ,
Lau KS,
Temporal recording of mammalian development and precancer
Nature.
2024
Oct
30;
634
(8036)
1187-1195
- PMID: 39478207 [PubMed]
2022
Overway E M,
Bosma K J,
Claxton D P,
Oeser J K,
Singh K,
Breidenbach L B,
Mchaourab H S,
Davis L K,
O'Brien R M,
Nonsynonymous single-nucleotide polymorphisms in the G6PC2 gene affect protein expression, enzyme activity, and fasting blood glucose
J Biol Chem.
J Biol Chem.
2022
Feb
298
(2)
101534
- PMID: 34954144 [PubMed]
- PMCID: PMC8800118
Del Alamo D,
Sala D,
Mchaourab HS,
Meiler J,
Sampling alternative conformational states of transporters and receptors with AlphaFold2
Elife.
2022
Mar
3;
11
(Online ahead of print)
e75751
- PMID: 35238773 [PubMed]
Claxton DP,
Overway EM,
Oeser JK,
O'Brien RM,
Mchaourab HS,
Biophysical and functional properties of purified glucose-6-phosphatase catalytic subunit 1
J Biol Chem.
2022
Jan
298
(1)
101520
- PMID: 34952005 [PubMed]
2021
Thaker TM,
Mishra S,
Zhou W,
Mohan M,
Tang Q,
Faraldo-Gomé J,
Mchaourab HS,
Tomasiak TM,
Asymmetric drug binding in an ATP-loaded inward-facing state of an ABC transporter
Nat Chem Biol.
2021
Dec
20;
(Online ahead of print)
- PMID: 34931066 [PubMed]
Schiemann O,
Heubach CA,
Abdullin D,
Ackermann K,
Azarkh M,
Bagryanskaya EG,
Drescher M,
Endeward B,
Freed JH,
Galazzo L,
Goldfarb D,
Hett T,
Hofer LE,
Ibáñez LF,
Hustedt EJ,
Kucher S,
Kuprov I,
Lovett JE,
Meyer A,
Ruthstein S,
Saxena S,
Stoll S,
Timmel CR,
Valentin MD,
Mchaourab HS,
Prisner TF,
Bode BE,
Bordignon E,
Bennati M,
Jeschke G,
Benchmark Test and Guidelines for DEER/PELDOR Experiments on Nitroxide-Labeled Biomolecules
J Am Chem Soc.
2021
Nov
3;
143
(43)
17875-17890
- PMID: 34664948 [PubMed]
Hustedt EJ,
Stein RA,
Mchaourab HS,
Protein functional dynamics from the rigorous global analysis of DEER data: Conditions, components, and conformations
J Gen Physiol.
2021
Nov
1;
53
(11)
e201711954
- PMID: 34529007 [PubMed]
- PMCID: PMC8449309
Del Alamo D,
Jagessar KL,
Meiler J,
Mchaourab HS,
Methodology for rigorous modeling of protein conformational changes by Rosetta using DEER distance restraints
PLoS Comput Biol.
2021
Jun
16;
7
(6)
e1009107
- PMID: 34133419 [PubMed]
- PMCID: PMC8238229
Aguilar J,
Cheng MH,
Font J,
Schwartz AC,
Ledwitch K,
Duran A,
Mabry SJ,
Belovich AN,
Zhu Y,
Carter AM,
Shi L,
Kurian MA,
Fenollar-Ferrer C,
Meiler J,
Ryan RM,
Mchaourab HS,
Bahar,
Matthies HJg,
Galli A,
Psychomotor impairments and therapeutic implications revealed by a mutation associated with infantile Parkinsonism-Dystonia
Elife.
2021
May
18;
10
(e68039)
- PMID: 34002696 [PubMed]
- PMCID: PMC8131106
Del Alamo D,
Govaerts C,
Mchaourab HS,
AlphaFold2 predicts the inward-facing conformation of the multidrug transporter LmrP
Proteins.
2021
May
11;
89
(9)
1226–1228
- PMID: 33973689 [PubMed]
- PMCID: PMC8522053
Claxton DP,
Jagessar KL,
Mchaourab HS,
Principles of Alternating Access in Multidrug and Toxin Extrusion (MATE) Transporters
J Mol Biol .
2021
Aug
6;
433
(16)
166959
- PMID: 33774036 [PubMed]
- PMCID: PMC8292195
Castellano S,
Claxton DP,
Ficici E,
Kusakizako T,
Stix R,
Zhou W,
Nureki O,
Mchaourab HS,
Faraldo-Gómez JD,
Conserved binding site in the N-lobe of prokaryotic MATE transporters suggests a role for Na + in ion-coupled drug efflux
J Biol Chem.
2021
Jan
5;
296
(100262)
- PMID: 33837745 [PubMed]
- PMCID: PMC7949106
2020
Han B,
Porta JC,
Hanks JL,
Peskova Y,
Binshtein E,
Dryden K,
Claxton DP,
Mchaourab HS,
Karakas E,
Ohi MD,
Kenworthy AK,
Structure and assembly of CAV1 8S complexes revealed by single particle electron microscopy
Sci Adv.
2020
Dec
2;
6
(49)
eabc6185
- PMID: 33268374 [PubMed]
Yardeni EH,
Mishra S,
Stein RA,
Bibi E,
Mchaourab HS,
The Multidrug Transporter MdfA Deviates from the Canonical Model of Alternating Access of MFS Transporters
J Mol Biol.
2020
Sep
18;
20
(432)
5665-5680
- PMID: 32860775 [PubMed]
- PMCID: PMC7541688
Rahman S,
Mchaourab HS,
ATP-dependent interactions of a cargo protein with the transmembrane domain of a polypeptide processing and secretion ABC transporter
J Biol Chem.
2020
Aug
20;
- PMID: 32820049 [PubMed]
Debruycker V,
Hutchin A,
Masureel M,
Masureel E,
Martens C,
Legrand P,
Stein RA,
Mchaourab HS,
Faraldo-Gómez JD,
Remaut H,
Govaerts C,
An embedded lipid in the multidrug transporter LmrP suggests a mechanism for polyspecificity
Nat Struct Mol Biol.
2020
Sep
27;
(9)
829-835
- PMID: 32719456 [PubMed]
Chavan S,
Cheng C,
Jiang T,
Mathews I,
Stein RA,
Koehl A,
Mchaourab HS,
Tajkhorshid E,
Maduke M,
A CLC-ec1 mutant reveals global conformational change and suggests a unifying mechanism for the CLC Cl -/H + transport cycle
Elife.
2020
Apr
20;
(9)
e53479
- PMID: 32310757 [PubMed]
- PMCID: PMC7253180
Yang Z,
Stein RA,
Ngendahimana T,
Pink M,
Rajca S,
Jeschke G,
Eaton S,
Mchaourab HS,
Rajca A,
Supramolecular Approach to Electron Paramagnetic Resonance Distance Measurement of Spin-Labeled Proteins
J Phys Chem B.
2020
Apr
23;
16
(124)
3291–3299
- PMID: 32227839 [PubMed]
Jagessar L,
Claxton P,
Stein RA,
Mchaourab HS,
Sequence and structural determinants of ligand-dependent alternating access of a MATE transporter
Proceedings of the National Academy of Sciences.
2020
Feb
19;
- PMID: 32075917 [PubMed]
Del Alamo D,
Tessemer H,
Stein RA,
Felix B,
Mchaourab HS,
Meiler J,
Rapid Simulation of Unprocessed DEER Decay Data for Protein Fold Prediction
Biophysical Journal.
2020
Jan
21;
2
(118)
366-375
- PMID: 31892409 [PubMed]
- PMCID: PMC6976798
2019
Yardeni E,
Bahrenberg T,
Stein RA,
Mishra S,
Zomot E,
Graham B,
Tuck K,
Huber T,
Bibi E,
Mchaourab HS,
Goldfarb D,
Probing the solution structure of the E. coli multidrug transporter MdfA using DEER distance measurements with nitroxide and Gd(III) spin labels
Scientific Reports .
2019
Aug
29;
9
(1)
12528
- PMID: 31467343 [PubMed]
- PMCID: PMC6715713
Dastvan R,
Mishra S,
Peskova YB,
Robert N,
Mchaourab HS,
Mechanism of allosteric modulation of P-glycoprotein by transport substrates and inhibitors
Science.
2019
May
17;
364
(6441)
689-692
- PMID: 31097669 [PubMed]
Campbell NG,
Shekar A,
Aguilar JI,
Peng D,
Navratna V,
Yang D,
Morley AN,
Duran AM,
Galli G,
O'Grady B,
Ramachandran R,
Sutcliffe JS,
Sitte HH,
Erreger K,
Meiler J,
Stockner T,
Bellan LM,
Matthies HJG,
Gouaux E,
Mchaourab HS,
Galli A,
Structural, functional, and behavioral insights of dopamine dysfunction revealed by a deletion in
Proceedings of the National Academy of Sciences of the United States of America.
2019
Feb
12;
116
(9)
3853-3862
- PMID: 30755521 [PubMed]
- PMCID: PMC6397532
2018
Paz A,
Claxton DP,
Kumar JP,
Kazmier K,
Bisignano P,
Sharma S,
Nolte SA,
Liwag TM,
Nayak V,
Wright EM,
Grabe M,
Mchaourab HS,
Abramson J,
Conformational transitions of the sodium-dependent sugar transporter, vSGLT
Proceedings of the National Academy of Sciences of the United States of America.
2018
Mar
05;
115
(12)
E2742-E2751
- PMID: 29507231 [PubMed]
- PMCID: PMC5866573
Song X,
Jensen MØ,
Jogini V,
Stein RA,
Lee CH,
Mchaourab HS,
Shaw DE,
Gouaux E,
Mechanism of NMDA receptor channel block by MK-801 and memantine
Nature.
2018
Apr
18;
556
(7702)
515-519
- PMID: 29670280 [PubMed]
- PMCID: PMC5962351
Claxton DP,
Jagessar KL,
Steed PR,
Stein RA,
Mchaourab HS,
Sodium and proton coupling in the conformational cycle of a MATE antiporter from
Proceedings of the National Academy of Sciences of the United States of America.
2018
Jun
18;
115
(27)
E6182-E6190
- PMID: 29915043 [PubMed]
- PMCID: PMC6142240
Mishra S,
Chandler SA,
Williams D,
Claxton DP,
Koteiche HA,
Stewart PL,
Benesch JLP,
Mchaourab HS,
Engineering of a Polydisperse Small Heat-Shock Protein Reveals Conserved Motifs of Oligomer Plasticity
Structure (London, England : 1993).
2018
Jul
05;
26
(8)
1116-1126.e4
- PMID: 29983375 [PubMed]
Warren GM,
Stein RA,
Mchaourab HS,
Eichman BF,
Movement of the RecG Motor Domain upon DNA Binding Is Required for Efficient Fork Reversal
International journal of molecular sciences.
2018
Oct
06;
19
(10)
- PMID: 30301235 [PubMed]
- PMCID: PMC6213257
Kusakizako T,
Claxton DP,
Tanaka Y,
Maturana AD,
Kuroda T,
Ishitani R,
Mchaourab HS,
Nureki O,
Structural Basis of H-Dependent Conformational Change in a Bacterial MATE Transporter
Structure (London, England : 1993).
2018
Nov
15;
27
(2)
293-301.e3
- PMID: 30449688 [PubMed]
Wu SY,
Zou P,
Mishra S,
Mchaourab HS,
Transgenic zebrafish models reveal distinct molecular mechanisms for cataract-linked αA-crystallin mutants
PloS one.
2018
Nov
26;
13
(11)
e0207540
- PMID: 30475834 [PubMed]
- PMCID: PMC6261105
2017
Verhalen B,
Dastvan R,
Thangapandian S,
Peskova Y,
Koteiche HA,
Nakamoto RK,
Tajkhorshid E,
Mchaourab HS,
Energy transduction and alternating access of the mammalian ABC transporter P-glycoprotein
Nature.
2017
Mar
13;
543
(7647)
738-741
- PMID: 28289287 [PubMed]
- PMCID: PMC5558441
Carra S,
Alberti S,
Arrigo PA,
Benesch JL,
Benjamin IJ,
Boelens W,
Bartelt-Kirbach B,
Brundel BJJM,
Buchner J,
Bukau B,
Carver JA,
Ecroyd H,
Emanuelsson C,
Finet S,
Golenhofen N,
Goloubinoff P,
Gusev N,
Haslbeck M,
Hightower LE,
Kampinga HH,
Klevit RE,
Liberek K,
Mchaourab HS,
McMenimen KA,
Poletti A,
Quinlan R,
Strelkov SV,
Toth ME,
Vierling E,
Tanguay RM,
The growing world of small heat shock proteins: from structure to functions
Cell stress & chaperones.
2017
Mar
31;
22
(4)
601-611
- PMID: 28364346 [PubMed]
- PMCID: PMC5465036
Collauto A,
Mishra S,
Litvinov A,
Mchaourab HS,
Goldfarb D,
Direct Spectroscopic Detection of ATP Turnover Reveals Mechanistic Divergence of ABC Exporters
Structure (London, England : 1993).
2017
Jul
14;
25
(8)
1264-1274.e3
- PMID: 28712805 [PubMed]
- PMCID: PMC5600498
Mishra S,
Wu SY,
Fuller AW,
Wang Z,
Rose KL,
Schey KL,
Mchaourab HS,
Loss of αB-crystallin function in zebrafish reveals critical roles in the development of the lens and stress resistance of the heart
The Journal of biological chemistry.
2017
Nov
21;
293
(2)
740-753
- PMID: 29162721 [PubMed]
- PMCID: PMC5767876
2016
Zhu S,
Stein RA,
Yoshioka C,
Lee CH,
Goehring A,
Mchaourab HS,
Gouaux E,
Mechanism of NMDA Receptor Inhibition and Activation.
Cell.
2016
Apr
6;
- PMID: 27062927 [PubMed]
Arrigoni C,
Rohaim A,
Shaya D,
Findeisen F,
Stein RA,
Nurva SR,
Mishra S,
Mchaourab HS,
Minor DL,
Unfolding of a Temperature-Sensitive Domain Controls Voltage-Gated Channel Activation.
Cell.
2016
Feb
25;
164
(5)
922-36
- PMID: 26919429 [PubMed]
- PMCID: PMC4769381
- NIHMSID: NIHMS757503
Khantwal CM,
Abraham SJ,
Han W,
Jiang T,
Chavan TS,
Cheng RC,
Elvington SM,
Liu CW,
Mathews II,
Stein RA,
Mchaourab HS,
Tajkhorshid E,
Maduke M,
Revealing an outward-facing open conformational state in a CLC Cl(-)/H(+) exchange transporter.
eLife.
2016
5
- PMID: 26799336 [PubMed]
- PMCID: PMC4769167
Dastvan R,
Fischer AW,
Mishra S,
Meiler J,
Mchaourab HS,
Protonation-dependent conformational dynamics of the multidrug transporter EmrE.
Proceedings of the National Academy of Sciences of the United States of America.
2016
Feb
2;
113
(5)
1220-5
- PMID: 26787875 [PubMed]
- PMCID: PMC4747756
Krug U,
Alexander NS,
Stein RA,
Keim A,
Mchaourab HS,
Sträter N,
Meiler J,
Characterization of the Domain Orientations of E. coli 5′-Nucleotidase by Fitting an Ensemble of Co…
Structure (London, England : 1993).
2016
Jan
5;
24
(1)
43-56
- PMID: 26724996 [PubMed]
- PMCID: PMC4706771
- NIHMSID: NIHMS744303
Martens C,
Stein RA,
Masureel M,
Roth A,
Mishra S,
Dawaliby R,
Konijnenberg A,
Sobott F,
Govaerts C,
Mchaourab HS,
Lipids modulate the conformational dynamics of a secondary multidrug transporter
Nature structural & molecular biology.
2016
Jul
11;
23
(8)
744-51
- PMID: 27399258 [PubMed]
- PMCID: PMC5248563
Wu SY,
Zou P,
Fuller AW,
Mishra S,
Wang Z,
Schey KL,
Mchaourab HS,
Expression of Cataract-linked γ-Crystallin Variants in Zebrafish Reveals a Proteostasis Network That Senses Protein Stability
The Journal of biological chemistry.
2016
Oct
21;
291
(49)
25387-25397
- PMID: 27770023 [PubMed]
- PMCID: PMC5207241
Kazmier K,
Claxton DP,
Mchaourab HS,
Alternating access mechanisms of LeuT-fold transporters: trailblazing towards the promised energy landscapes
Current opinion in structural biology.
2016
Dec
30;
45
100-108
- PMID: 28040635 [PubMed]
- PMCID: PMC5491374
2015
Koteiche HA,
Claxton DP,
Mishra S,
Stein RA,
McDonald ET,
Mchaourab HS,
Species-Specific Structural and Functional Divergence of α-Crystallins: Zebrafish αBa- and Rodent …
Biochemistry.
2015
Sep
29;
54
(38)
5949-58
- PMID: 26378715 [PubMed]
Zou P,
Wu SY,
Koteiche HA,
Mishra S,
Levic DS,
Knapik E,
Chen W,
Mchaourab HS,
A conserved role of αA-crystallin in the development of the zebrafish embryonic lens.
Experimental eye research.
2015
Sep
138
104-13
- PMID: 26149094 [PubMed]
- PMCID: PMC4638411
- NIHMSID: NIHMS708963
Claxton DP,
Kazmier K,
Mishra S,
Mchaourab HS,
Navigating Membrane Protein Structure, Dynamics, and Energy Landscapes Using Spin Labeling and EPR S…
Methods in enzymology.
2015
564
349-87
- PMID: 26477257 [PubMed]
Mori T,
Mchaourab H,
Johnson CH,
Circadian Clocks: Unexpected Biochemical Cogs.
Current biology : CB.
2015
Oct
5;
25
(19)
R842-4
- PMID: 26439342 [PubMed]
- PMCID: PMC4697453
- NIHMSID: NIHMS744952
Schmandt N,
Velisetty P,
Chalamalasetti SV,
Stein RA,
Bonner R,
Talley L,
Parker MD,
Mchaourab HS,
Yee VC,
Lodowski DT,
Chakrapani S,
A chimeric prokaryotic pentameric ligand-gated channel reveals distinct pathways of activation.
The Journal of general physiology.
2015
Oct
146
(4)
323-40
- PMID: 26415570 [PubMed]
- PMCID: PMC4586589
Kaur H,
Lakatos A,
Spadaccini R,
Vogel R,
Hoffmann C,
Becker-Baldus J,
Ouari O,
Tordo P,
Mchaourab H,
Glaubitz C,
The ABC exporter MsbA probed by solid state NMR – challenges and opportunities.
Biological chemistry.
2015
Sep
396
(9-10)
1135-49
- PMID: 25849794 [PubMed]
Meyer V,
Swanson MA,
Clouston LJ,
Boratyński PJ,
Stein RA,
Mchaourab HS,
Rajca A,
Eaton SS,
Eaton GR,
Room-temperature distance measurements of immobilized spin-labeled protein by DEER/PELDOR.
Biophysical journal.
2015
Mar
10;
108
(5)
1213-9
- PMID: 25762332 [PubMed]
- PMCID: PMC4375670
Anderson DM,
Floyd KA,
Barnes S,
Clark JM,
Clark JI,
Mchaourab H,
Schey KL,
A method to prevent protein delocalization in imaging mass spectrometry of non-adherent tissues: app…
Analytical and bioanalytical chemistry.
2015
Mar
407
(8)
2311-20
- PMID: 25665708 [PubMed]
- PMCID: PMC4379205
- NIHMSID: NIHMS662674
2014
Kazmier K,
Sharma S,
Islam SM,
Roux B,
Mchaourab HS,
Conformational cycle and ion-coupling mechanism of the Na+/hydantoin transporter Mhp1.
Proceedings of the National Academy of Sciences of the United States of America.
2014
Oct
14;
111
(41)
14752-7
- PMID: 25267652 [PubMed]
- PMCID: PMC4205665
Yu Z,
Quine RW,
Rinard GA,
Tseitlin M,
Elajaili H,
Kathirvelu V,
Clouston LJ,
Boratyński PJ,
Rajca A,
Stein R,
Mchaourab H,
Eaton SS,
Eaton GR,
Rapid-scan EPR of immobilized nitroxides.
Journal of magnetic resonance (San Diego, Calif. : 1997).
2014
Oct
247
67-71
- PMID: 25240151 [PubMed]
- PMCID: PMC4247172
- NIHMSID: NIHMS626711
Dürr KL,
Chen L,
Stein RA,
De Zorzi R,
Folea IM,
Walz T,
Mchaourab HS,
Gouaux E,
Structure and dynamics of AMPA receptor GluA2 in resting, pre-open, and desensitized states.
Cell.
2014
Aug
14;
158
(4)
778-92
- PMID: 25109876 [PubMed]
- PMCID: PMC4263325
- NIHMSID: NIHMS620755
Mishra S,
Verhalen B,
Stein RA,
Wen PC,
Tajkhorshid E,
Mchaourab HS,
Conformational dynamics of the nucleotide binding domains and the power stroke of a heterodimeric AB…
eLife.
2014
3
e02740
- PMID: 24837547 [PubMed]
- PMCID: PMC4046567
Kazmier K,
Sharma S,
Quick M,
Islam SM,
Roux B,
Weinstein H,
Javitch JA,
McHaourab HS,
Conformational dynamics of ligand-dependent alternating access in LeuT.
Nature structural & molecular biology.
2014
May
21
(5)
472-9
- PMID: 24747939 [PubMed]
- PMCID: PMC4050370
- NIHMSID: NIHMS580423
Masureel M,
Martens C,
Stein RA,
Mishra S,
Ruysschaert JM,
Mchaourab HS,
Govaerts C,
Protonation drives the conformational switch in the multidrug transporter LmrP.
Nature chemical biology.
2014
Feb
10
(2)
149-55
- PMID: 24316739 [PubMed]
- PMCID: PMC4749020
- NIHMSID: NIHMS756889
2013
Hamilton PJ,
Campbell NG,
Sharma S,
Erreger K,
Herborg Hansen F,
Saunders C,
Belovich AN,
,
Sahai MA,
Cook EH,
Gether U,
McHaourab HS,
Matthies HJ,
Sutcliffe JS,
Galli A,
De novo mutation in the dopamine transporter gene associates dopamine dysfunction with autism spectr…
Molecular psychiatry.
2013
Dec
18
(12)
1315-23
- PMID: 23979605 [PubMed]
- PMCID: PMC4046646
- NIHMSID: NIHMS569876
Steed PR,
Stein RA,
Mishra S,
Goodman MC,
McHaourab HS,
Na⁺-substrate coupling in the multidrug antiporter norm probed with a spin-labeled substrate.
Biochemistry.
2013
Aug
27;
52
(34)
5790-9
- PMID: 23902581 [PubMed]
- PMCID: PMC3842230
- NIHMSID: NIHMS525263
Wen PC,
Verhalen B,
Wilkens S,
Mchaourab HS,
Tajkhorshid E,
On the origin of large flexibility of P-glycoprotein in the inward-facing state.
The Journal of biological chemistry.
2013
Jun
28;
288
(26)
19211-20
- PMID: 23658020 [PubMed]
- PMCID: PMC3696692
Islam SM,
Stein RA,
McHaourab HS,
Roux B,
Structural refinement from restrained-ensemble simulations based on EPR/DEER data: application to T4…
The journal of physical chemistry. B.
2013
May
2;
117
(17)
4740-54
- PMID: 23510103 [PubMed]
- PMCID: PMC3684008
- NIHMSID: NIHMS467321
Shi J,
Koteiche HA,
McDonald ET,
Fox TL,
Stewart PL,
McHaourab HS,
Cryoelectron microscopy analysis of small heat shock protein 16.5 (Hsp16.5) complexes with T4 lysozy…
The Journal of biological chemistry.
2013
Feb
15;
288
(7)
4819-30
- PMID: 23277356 [PubMed]
- PMCID: PMC3576087
Hamilton PJ,
Campbell NG,
Sharma S,
Erreger K,
Hansen FH,
Saunders C,
Belovich AN,
Sahai MA,
Cook EH,
Gether U,
McHaourab HS,
Matthies HJ,
Sutcliffe JS,
Galli A,
Drosophila melanogaster: a novel animal model for the behavioral characterization of autism-associat…
Molecular psychiatry.
2013
Dec
18
(12)
1235
- PMID: 24253181 [PubMed]
Steed PR,
Zou P,
Trone KE,
Mchaourab HS,
Structure and pH-induced structural rearrangements of the putative multidrug efflux pump EmrD in lip…
Biochemistry.
2013
Nov
12;
52
(45)
7964-74
- PMID: 24148002 [PubMed]
- PMCID: PMC3855667
- NIHMSID: NIHMS535461
Alexander NS,
Stein RA,
Koteiche HA,
Kaufmann KW,
McHaourab HS,
Meiler J,
RosettaEPR: rotamer library for spin label structure and dynamics.
PloS one.
2013
8
(9)
e72851
- PMID: 24039810 [PubMed]
- PMCID: PMC3764097
2012
Mishra S,
Stein RA,
McHaourab HS,
Cataract-linked γD-crystallin mutants have weak affinity to lens chaperones α-crystallins.
FEBS letters.
2012
Feb
17;
586
(4)
330-6
- PMID: 22289178 [PubMed]
- PMCID: PMC3282170
- NIHMSID: NIHMS353137
Mishra S,
Stein RA,
Mchaourab HS,
Cataract-linked γD-crystallin mutants have weak affinity to lens chaperones α-crystallins
FEBS Letters.
2012
Jan
29;
4
(586)
330-6
Yirdaw RB,
McHaourab HS,
Direct observation of T4 lysozyme hinge-bending motion by fluorescence correlation spectroscopy.
Biophysical journal.
2012
Oct
3;
103
(7)
1525-36
- PMID: 23062345 [PubMed]
- PMCID: PMC3471472
McHaourab HS,
Lin YL,
Spiller BW,
Crystal structure of an activated variant of small heat shock protein Hsp16.5.
Biochemistry.
2012
Jun
26;
51
(25)
5105-12
- PMID: 22670769 [PubMed]
- PMCID: PMC3384710
- NIHMSID: NIHMS384778
McDonald ET,
Bortolus M,
Koteiche HA,
Mchaourab HS,
Sequence, structure, and dynamic determinants of Hsp27 (HspB1) equilibrium dissociation are encoded …
Biochemistry.
2012
Feb
14;
51
(6)
1257-68
- PMID: 22264079 [PubMed]
- PMCID: PMC3293247
- NIHMSID: NIHMS354225
2011
Hoffman L,
Stein RA,
Colbran RJ,
Mchaourab HS,
Conformational changes underlying calcium/calmodulin-dependent protein kinase II activation.
The EMBO journal.
2011
Apr
6;
30
(7)
1251-62
- PMID: 21343908 [PubMed]
- PMCID: PMC3094114
Kazmier K,
Alexander NS,
Meiler J,
McHaourab HS,
Algorithm for selection of optimized EPR distance restraints for de novo protein structure determina…
Journal of structural biology.
2011
Mar
173
(3)
549-57
- PMID: 21074624 [PubMed]
- PMCID: PMC3073550
- NIHMSID: NIHMS252690
Hirst SJ,
Alexander N,
McHaourab HS,
Meiler J,
RosettaEPR: an integrated tool for protein structure determination from sparse EPR data.
Journal of structural biology.
2011
Mar
173
(3)
506-14
- PMID: 21029778 [PubMed]
- PMCID: PMC3040274
- NIHMSID: NIHMS249055
McHaourab HS,
Steed PR,
Kazmier K,
Toward the fourth dimension of membrane protein structure: insight into dynamics from spin-labeling …
Structure (London, England : 1993).
2011
Nov
9;
19
(11)
1549-61
- PMID: 22078555 [PubMed]
- PMCID: PMC3224804
- NIHMSID: NIHMS335515
Mushrush DJ,
Koteiche HA,
Sammons MA,
Link AJ,
McHaourab HS,
Lacy DB,
Studies of the mechanistic details of the pH-dependent association of botulinum neurotoxin with memb…
The Journal of biological chemistry.
2011
Jul
29;
286
(30)
27011-8
- PMID: 21652698 [PubMed]
- PMCID: PMC3143659
2010
Tycko R,
Savtchenko R,
Ostapchenko VG,
Makarava N,
Baskakov IV,
The α-helical C-terminal domain of full-length recombinant PrP converts to an in-register parallel …
Biochemistry.
2010
Nov
9;
49
(44)
9488-97
- PMID: 20925423 [PubMed]
- PMCID: PMC3025268
- NIHMSID: NIHMS244969
Qin X,
Byrne M,
Mori T,
Zou P,
Williams DR,
McHaourab H,
Johnson CH,
Intermolecular associations determine the dynamics of the circadian KaiABC oscillator.
Proceedings of the National Academy of Sciences of the United States of America.
2010
Aug
17;
107
(33)
14805-10
- PMID: 20679240 [PubMed]
- PMCID: PMC2930409
Claxton DP,
Quick M,
Shi L,
de Carvalho FD,
Weinstein H,
Javitch JA,
McHaourab HS,
Ion/substrate-dependent conformational dynamics of a bacterial homolog of neurotransmitter:sodium sy…
Nature structural & molecular biology.
2010
Jul
17
(7)
822-9
- PMID: 20562855 [PubMed]
- PMCID: PMC3245867
- NIHMSID: NIHMS336414
Amadi ST,
Koteiche HA,
Mishra S,
McHaourab HS,
Structure, dynamics, and substrate-induced conformational changes of the multidrug transporter EmrE …
The Journal of biological chemistry.
2010
Aug
20;
285
(34)
26710-8
- PMID: 20551331 [PubMed]
- PMCID: PMC2924113
Zou P,
McHaourab HS,
Increased sensitivity and extended range of distance measurements in spin-labeled membrane proteins:…
Biophysical journal.
2010
Mar
17;
98
(6)
L18-20
- PMID: 20303847 [PubMed]
- PMCID: PMC2849076
Gustot A,
Smriti,
Ruysschaert JM,
McHaourab H,
Govaerts C,
Lipid composition regulates the orientation of transmembrane helices in HorA, an ABC multidrug trans…
The Journal of biological chemistry.
2010
May
7;
285
(19)
14144-51
- PMID: 20223819 [PubMed]
- PMCID: PMC2863179
2009
Zou P,
Bortolus M,
McHaourab HS,
Conformational cycle of the ABC transporter MsbA in liposomes: detailed analysis using double electr…
Journal of molecular biology.
2009
Oct
30;
393
(3)
586-97
- PMID: 19715702 [PubMed]
- PMCID: PMC2760609
- NIHMSID: NIHMS142434
McHaourab HS,
Godar JA,
Stewart PL,
Structure and mechanism of protein stability sensors: chaperone activity of small heat shock protein…
Biochemistry.
2009
May
12;
48
(18)
3828-37
- PMID: 19323523 [PubMed]
- PMCID: PMC2785012
- NIHMSID: NIHMS107671
Smriti P,
Zou HS,
McHaourab,
Mapping daunorubicin-binding Sites in the ATP-binding cassette transporter MsbA using site-specific …
The Journal of biological chemistry.
2009
May
15;
284
(20)
13904-13
- PMID: 19278995 [PubMed]
- PMCID: PMC2679490
Latham JC,
Stein RA,
Bornhop DJ,
Mchaourab HS,
Free-solution label-free detection of alpha-crystallin chaperone interactions by back-scattering int…
Analytical chemistry.
2009
Mar
1;
81
(5)
1865-71
- PMID: 19178288 [PubMed]
- PMCID: PMC2787765
- NIHMSID: NIHMS91945
Kumar MS,
Koteiche HA,
Claxton DP,
Mchaourab HS,
Disulfide cross-links in the interaction of a cataract-linked alphaA-crystallin mutant with betaB1-c…
FEBS letters.
2009
Jan
5;
583
(1)
175-9
- PMID: 19071118 [PubMed]
Zou P,
McHaourab HS,
Alternating access of the putative substrate-binding chamber in the ABC transporter MsbA.
Journal of molecular biology.
2009
Oct
30;
393
(3)
574-85
- PMID: 19715704 [PubMed]
- PMCID: PMC2760602
- NIHMSID: NIHMS142437
2008
McHaourab HS,
Mishra S,
Koteiche HA,
Amadi SH,
Role of sequence bias in the topology of the multidrug transporter EmrE.
Biochemistry.
2008
Aug
5;
47
(31)
7980-2
- PMID: 18616286 [PubMed]
Alexander N,
Bortolus M,
Al-Mestarihi A,
Mchaourab H,
Meiler J,
De novo high-resolution protein structure determination from sparse spin-labeling EPR data.
Structure (London, England : 1993).
2008
Feb
16
(2)
181-95
- PMID: 18275810 [PubMed]
- PMCID: PMC2390841
- NIHMSID: NIHMS40895
Claxton DP,
Zou P,
Mchaourab HS,
Structure and orientation of T4 lysozyme bound to the small heat shock protein alpha-crystallin.
Journal of molecular biology.
2008
Jan
25;
375
(4)
1026-39
- PMID: 18062989 [PubMed]
- PMCID: PMC2276617
- NIHMSID: NIHMS38818
2007
McHaourab HS,
Kumar MS,
Koteiche HA,
Specificity of alphaA-crystallin binding to destabilized mutants of betaB1-crystallin.
FEBS letters.
2007
May
15;
581
(10)
1939-43
- PMID: 17449033 [PubMed]
- PMCID: PMC2219212
- NIHMSID: NIHMS23345
Koteiche HA,
Kumar MS,
McHaourab HS,
Analysis of betaB1-crystallin unfolding equilibrium by spin and fluorescence labeling: evidence of a…
FEBS letters.
2007
May
15;
581
(10)
1933-8
- PMID: 17448466 [PubMed]
- PMCID: PMC2394508
- NIHMSID: NIHMS23340
Mori T,
Williams DR,
Byrne MO,
Qin X,
Egli M,
Mchaourab HS,
Stewart PL,
Johnson CH,
Elucidating the ticking of an in vitro circadian clockwork.
PLoS biology.
2007
Apr
5
(4)
e93
- PMID: 17388688 [PubMed]
- PMCID: PMC1831719
Zou P,
Surendhran K,
Mchaourab HS,
Distance measurements by fluorescence energy homotransfer: evaluation in T4 lysozyme and correlation…
Biophysical journal.
2007
Feb
15;
92
(4)
L27-9
- PMID: 17142264 [PubMed]
- PMCID: PMC1783887
Cobb NJ,
Sönnichsen FD,
McHaourab H,
Surewicz WK,
Molecular architecture of human prion protein amyloid: a parallel, in-register beta-structure.
Proceedings of the National Academy of Sciences of the United States of America.
2007
Nov
27;
104
(48)
18946-51
- PMID: 18025469 [PubMed]
- PMCID: PMC2141888
Borbat PP,
Surendhran K,
Bortolus M,
Zou P,
Freed JH,
Mchaourab HS,
Conformational motion of the ABC transporter MsbA induced by ATP hydrolysis.
PLoS biology.
2007
Oct
5
(10)
e271
- PMID: 17927448 [PubMed]
- PMCID: PMC2001213
2006
Shi J,
Koteiche HA,
McHaourab HS,
Stewart PL,
Cryoelectron microscopy and EPR analysis of engineered symmetric and polydisperse Hsp16.5 assemblies…
The Journal of biological chemistry.
2006
Dec
29;
281
(52)
40420-8
- PMID: 17079234 [PubMed]
Koteiche HA,
Mchaourab HS,
Mechanism of a hereditary cataract phenotype. Mutations in alphaA-crystallin activate substrate bind…
The Journal of biological chemistry.
2006
May
19;
281
(20)
14273-9
- PMID: 16531622 [PubMed]
2005
Koteiche HA,
Chiu S,
Majdoch RL,
Stewart PL,
Mchaourab HS,
Atomic models by cryo-EM and site-directed spin labeling: application to the N-terminal region of Hs…
Structure (London, England : 1993).
2005
Aug
13
(8)
1165-71
- PMID: 16084388 [PubMed]
Altenbach C,
Froncisz W,
Hemker R,
McHaourab H,
Hubbell WL,
Accessibility of nitroxide side chains: absolute Heisenberg exchange rates from power saturation EPR…
Biophysical journal.
2005
Sep
89
(3)
2103-12
- PMID: 15994891 [PubMed]
- PMCID: PMC1366712
Dong J,
Yang G,
McHaourab HS,
Structural basis of energy transduction in the transport cycle of MsbA.
Science (New York, N.Y.).
2005
May
13;
308
(5724)
1023-8
- PMID: 15890883 [PubMed]
Shashidharamurthy R,
Koteiche HA,
Dong J,
McHaourab HS,
Mechanism of chaperone function in small heat shock proteins: dissociation of the HSP27 oligomer is …
The Journal of biological chemistry.
2005
Feb
18;
280
(7)
5281-9
- PMID: 15542604 [PubMed]
2004
Sathish HA,
Koteiche HA,
McHaourab HS,
Binding of destabilized betaB2-crystallin mutants to alpha-crystallin: the role of a folding interme…
The Journal of biological chemistry.
2004
Apr
16;
279
(16)
16425-32
- PMID: 14761939 [PubMed]
2003
Sathish HA,
Stein RA,
Yang G,
Mchaourab HS,
Mechanism of chaperone function in small heat-shock proteins. Fluorescence studies of the conformati…
The Journal of biological chemistry.
2003
Nov
7;
278
(45)
44214-21
- PMID: 12928430 [PubMed]
Koteiche HA,
Reeves MD,
McHaourab HS,
Structure of the substrate binding pocket of the multidrug transporter EmrE: site-directed spin labe…
Biochemistry.
2003
May
27;
42
(20)
6099-105
- PMID: 12755611 [PubMed]
Koteiche HA,
McHaourab HS,
Mechanism of chaperone function in small heat-shock proteins. Phosphorylation-induced activation of …
The Journal of biological chemistry.
2003
Mar
21;
278
(12)
10361-7
- PMID: 12529319 [PubMed]
2002
Mchaourab HS,
Dodson EK,
Koteiche HA,
Mechanism of chaperone function in small heat shock proteins. Two-mode binding of the excited states…
The Journal of biological chemistry.
2002
Oct
25;
277
(43)
40557-66
- PMID: 12189146 [PubMed]
Koteiche HA,
Mchaourab HS,
The determinants of the oligomeric structure in Hsp16.5 are encoded in the alpha-crystallin domain.
FEBS letters.
2002
May
22;
519
(1-3)
16-22
- PMID: 12023011 [PubMed]
Borbat PP,
McHaourab HS,
Freed JH,
Protein structure determination using long-distance constraints from double-quantum coherence ESR: s…
Journal of the American Chemical Society.
2002
May
15;
124
(19)
5304-14
- PMID: 11996571 [PubMed]
Mansoor SE,
McHaourab HS,
Farrens DL,
Mapping proximity within proteins using fluorescence spectroscopy. A study of T4 lysozyme showing th…
Biochemistry.
2002
Feb
26;
41
(8)
2475-84
- PMID: 11851393 [PubMed]
2000
Bova MP,
McHaourab HS,
Han Y,
Fung BK,
Subunit exchange of small heat shock proteins. Analysis of oligomer formation of alphaA-crystallin a…
The Journal of biological chemistry.
2000
Jan
14;
275
(2)
1035-42
- PMID: 10625643 [PubMed]
Kaplan RS,
Mayor JA,
Kotaria R,
Walters DE,
McHaourab HS,
The yeast mitochondrial citrate transport protein: determination of secondary structure and solvent …
Biochemistry.
2000
Aug
8;
39
(31)
9157-63
- PMID: 10924109 [PubMed]
Haley DA,
Bova MP,
Huang QL,
Mchaourab HS,
Stewart PL,
Small heat-shock protein structures reveal a continuum from symmetric to variable assemblies.
Journal of molecular biology.
2000
Apr
28;
298
(2)
261-72
- PMID: 10764595 [PubMed]
1999
Koteiche HA,
Mchaourab HS,
Folding pattern of the alpha-crystallin domain in alphaA-crystallin determined by site-directed spin…
Journal of molecular biology.
1999
Nov
26;
294
(2)
561-77
- PMID: 10610780 [PubMed]
Mansoor SE,
McHaourab HS,
Farrens DL,
Determination of protein secondary structure and solvent accessibility using site-directed fluoresce…
Biochemistry.
1999
Dec
7;
38
(49)
16383-93
- PMID: 10587464 [PubMed]
Barnes JP,
Liang Z,
Mchaourab HS,
Freed JH,
Hubbell WL,
A multifrequency electron spin resonance study of T4 lysozyme dynamics.
Biophysical journal.
1999
Jun
76
(6)
3298-306
- PMID: 10354455 [PubMed]
- PMCID: PMC1300299
Mchaourab HS,
Kálai T,
Hideg K,
Hubbell WL,
Motion of spin-labeled side chains in T4 lysozyme: effect of side chain structure.
Biochemistry.
1999
Mar
9;
38
(10)
2947-55
- PMID: 10074347 [PubMed]
Berengian AR,
Parfenova M,
Mchaourab HS,
Site-directed spin labeling study of subunit interactions in the alpha-crystallin domain of small he…
The Journal of biological chemistry.
1999
Mar
5;
274
(10)
6305-14
- PMID: 10037719 [PubMed]
1998
Koteiche HA,
Berengian AR,
Mchaourab HS,
Identification of protein folding patterns using site-directed spin labeling. Structural characteriz…
Biochemistry.
1998
Sep
15;
37
(37)
12681-8
- PMID: 9737844 [PubMed]
1997
Mchaourab HS,
Oh KJ,
Fang CJ,
Hubbell WL,
Conformation of T4 lysozyme in solution. Hinge-bending motion and the substrate-induced conformation…
Biochemistry.
1997
Jan
14;
36
(2)
307-16
- PMID: 9003182 [PubMed]
Mchaourab HS,
Berengian AR,
Koteiche HA,
Site-directed spin-labeling study of the structure and subunit interactions along a conserved sequen…
Biochemistry.
1997
Dec
2;
36
(48)
14627-34
- PMID: 9398181 [PubMed]
Berengian AR,
Bova MP,
Mchaourab HS,
Structure and function of the conserved domain in alphaA-crystallin. Site-directed spin labeling ide…
Biochemistry.
1997
Aug
19;
36
(33)
9951-7
- PMID: 9296605 [PubMed]
Qu K,
Vaughn JL,
Sienkiewicz A,
Scholes CP,
Fetrow JS,
Kinetics and motional dynamics of spin-labeled yeast iso-1-cytochrome c: 1. Stopped-flow electron pa…
Biochemistry.
1997
Mar
11;
36
(10)
2884-97
- PMID: 9062118 [PubMed]
1996
Hubbell WL,
Mchaourab HS,
Altenbach C,
Lietzow MA,
Watching proteins move using site-directed spin labeling.
Structure (London, England : 1993).
1996
Jul
15;
4
(7)
779-83
- PMID: 8805569 [PubMed]
Mchaourab HS,
Lietzow MA,
Hideg K,
Hubbell WL,
Motion of spin-labeled side chains in T4 lysozyme. Correlation with protein structure and dynamics.
Biochemistry.
1996
Jun
18;
35
(24)
7692-704
- PMID: 8672470 [PubMed]
1995
Singh RJ,
Feix JB,
Mchaourab HS,
Hogg N,
Kalyanaraman B,
Spin-labeling study of the oxidative damage to low-density lipoprotein.
Archives of biochemistry and biophysics.
1995
Jun
20;
320
(1)
155-61
- PMID: 7793976 [PubMed]
1994
Singh RJ,
Hogg N,
Mchaourab HS,
Kalyanaraman B,
Physical and chemical interactions between nitric oxide and nitroxides.
Biochimica et biophysica acta.
1994
Dec
15;
1201
(3)
437-41
- PMID: 7803474 [PubMed]
Mchaourab HS,
Hyde JS,
Feix JB,
Binding and state of aggregation of spin-labeled cecropin AD in phospholipid bilayers: effects of su…
Biochemistry.
1994
May
31;
33
(21)
6691-9
- PMID: 8204604 [PubMed]
1993
Mchaourab HS,
Hyde JS,
Feix JB,
Aggregation state of spin-labeled cecropin AD in solution.
Biochemistry.
1993
Nov
9;
32
(44)
11895-902
- PMID: 7692965 [PubMed]
Mchaourab HS,
Pfenninger S,
Antholine WE,
Felix CC,
Hyde JS,
Kroneck PM,
Multiquantum EPR of the mixed valence copper site in nitrous oxide reductase.
Biophysical journal.
1993
May
64
(5)
1576-9
- PMID: 8391869 [PubMed]
- PMCID: PMC1262484