>

Fine-Needle Aspiration-Based Patient-Derived Cancer Organoids

Authors

Vilgelm AE Anne , Bergdorf K Kensey , Wolf M Melissa , Bharti V Vijaya , Shattuck-Brandt R Rebecca , Blevins A Ashlyn , Jones C Caroline , Phifer C Courtney , Lee MA Mason , Lowe C Cindy , Rachel H Hongo , Boyd K Kelli , James N Netterville , Rohde S Sara , Idrees K Kamran , Bauer JA Joshua , Westover D David , Reinfeld B Bradley , Baregamian N Naira , Richmond A Ann , Rathmell K Kimryn , Lee E Ethan , McDonald OG Oliver , Weiss VL Vivian .
iScience. 2020 8 21; 8(23).

Abstract

Patient-derived cancer organoids hold great potential to accurately model and predict therapeutic responses. Efficient organoid isolation methods that mini- mize post-collection manipulation of tissues would improve adaptability, accu- racy, and applicability to both experimental and real-time clinical settings. Here we present a simple and minimally invasive fine-needle aspiration (FNA)-based or- ganoid culture technique using a variety of tumor types including gastrointes- tinal, thyroid, melanoma, and kidney. This method isolates organoids directly from patients at the bedside or from resected tissues, requiring minimal tissue processing while preserving the histologic growth patterns and infiltrating im- mune cells. Finally, we illustrate diverse downstream applications of this tech- nique including in vitro high-throughput chemotherapeutic screens, in situ im- mune cell characterization, and in vivo patient-derived xenografts. Thus, routine clinical FNA-based collection techniques represent an unappreciated sub- stantial source of material that can be exploited to generate tumor organoids from a variety of tumor types for both discovery and clinical applications.