Frame from a movie of an A7r5 cell expressing GFP-Src. Here, GFP-Src can been seen in vesicles and membrane tubules, which are transported along MTs to and from the cell periphery. GFP-Src is shown in green and the MT network is highlighted by mCherry-EMTB, which is shown in red.
Image showing two distinct localizations of the MT-binding protein RASSF1A: segmental RASSF1A localization to peripheral MTs (indicated by white arrow), and RASSF1A-bound Golgi-derived MTs (indicated by yellow arrows). The Golgi is shown in green, RFP-RASSF1A is shown in red.
Image showing the microtubule cytoskeleton (red), the Golgi (green) and nuclei (blue) in a Retinal Pigment Epithelial cell (RPE-1).
A7r5 rat aortic smooth muscle cells transfected with constituvely active Src to induce podosome formation at the cell periphery. In this image, bright actin puncta highlight the dense actin-core of the podosome. Phospho-Src (Tyr416) is shown in red, actin is shown in green (Phalloidin).
[Project 1] MT nucleation at MTOCs after short ice recovery in an RPE cell. White arrow indicates centrosomal nucleation, yellow arrowheads indicate MTs nucleated at the Golgi. MTs are shown in red, the Golgi is shown in blue, centrosomes are highlighted by GFP-centrin.
Image of an A7r5 cell, which has been depleted of CLASPs. In the absence of MT-stabilizing CLASP proteins, the MT network is less dense and disorganized. MTs are shown in red, the nucleus is shown in blue (DAPI).
A dividing cell in a cultered MDCK monolayer. MTs are shown in red, centrosomes are shown in green.
Image of a pancreatic islet in which the β-cells have well developed Golgi complexes. MTs are shown in red, the Golgi is shown in green.
MT nucleation at MTOCs after short ice recovery in an RPE cell. Short, newly-formed MTs can be seen at the centrosome as well as at Golgi mini-stacks. MTs are shown in red, the Golgi is shown in blue, centrosomes are highlighted by GFP-centrin.
Electron micrograph (EM) depicting the cytoskeleton of a non-targeted control A7r5 cell. The image shows actin bundles as well as individual MTs.
[Project 2] Image of an RPE cell in interphase with a compact and asymmetrical Golgi complex. MTs are shown in red, the Golgi is shown in cyan, and the centrosomes are labeled with GFP-centrin.
Image showing the microtubule cytoskeleton (red), the Golgi (green) and nuclei (blue) in a Retinal Pigment Epithelial cell (RPE-1).
MT nucleation at MTOCs after short ice recovery in an RPE cell undergoing telophase. Short, newly-formed MTs can be seen at the centrosome as well as at Golgi mini-stacks. MTs are shown in red, the Golgi is shown in blue, centrosomes are highlighted by GFP-centrin.
Fucci cycle indicator was used to exhibit that as cell cycle progresses from G1 (red) to S-phase (yellow) to G2 (green), the Golgi complex (white) localizes around the equator of the nucleus.
MTs in a kinesore (kinesin-1 modulator)-treated RPE cell
Time lapse image of Golgi cargo (ESCargo) in an RPE cell
Mouse islet stained for insulin (red) and tubulin (green)
MT directionality (green indicates parallel to the membrane, red indicates perpendicular to the membrane) in MIN6 beta cell
MT (green) sliding demonstrated using photoconvertible probe (red)