Site identification in high-throughput RNA-protein interaction data.
Authors
Uren
PJ
Philip J
,
Bahrami-Samani
E
Emad
,
Burns
SC
Suzanne C
,
Qiao
M
Mei
,
Karginov
FV
Fedor V
,
Hodges
E
Emily
,
Hannon
GJ
Gregory J
,
Sanford
JR
Jeremy R
,
Penalva
LO
Luiz O F
,
Smith
AD
Andrew D
.
Bioinformatics (Oxford, England). 2012 12 1; 28(23).
3013-20
Bioinformatics (Oxford, England). 2012 12 1; 28(23).
3013-20
Abstract
Post-transcriptional and co-transcriptional regulation is a crucial link between genotype and phenotype. The central players are the RNA-binding proteins, and experimental technologies [such as cross-linking with immunoprecipitation- (CLIP-) and RIP-seq] for probing their activities have advanced rapidly over the course of the past decade. Statistically robust, flexible computational methods for binding site identification from high-throughput immunoprecipitation assays are largely lacking however.