{"id":143,"date":"2018-05-24T15:28:22","date_gmt":"2018-05-24T15:28:22","guid":{"rendered":"https:\/\/lab.dev.vanderbilt.edu\/cdb-core-lab\/qsonica-q500-sonicator\/"},"modified":"2021-03-09T17:13:23","modified_gmt":"2021-03-09T17:13:23","slug":"qsonica-q500-sonicator","status":"publish","type":"page","link":"https:\/\/lab.dev.vanderbilt.edu\/cdb-core-lab\/qsonica-q500-sonicator\/","title":{"rendered":"Qsonica Q500 Sonicator"},"content":{"rendered":"

Location: 4141 MRB3<\/strong><\/h2>\n

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*Note: There is mandatory training by Equipment Manager for all new users prior to use.<\/strong><\/h4>\n

Safety<\/h3>\n

Ensure that door is properly closed on noise-proof cabinet prior to starting sonicator. If you need to open the cabinet door while sonicator is turned on, be sure to wear ear protection for yourself and anyone else in the room and close the doors to the room out of consideration for others.<\/p>\n

DO NOT allow the horn or micro tip to contact lab stands, beakers, etc. or horn\/micro tip failure may result.<\/p>\n

DO NOT touch the horn or tip when ultrasonics are active. When handling, removing, or attaching a tool or tip, be sure that the ultrasonic processor is powered OFF. Touching the tool while the unit is on can result in serious personal injury.<\/p>\n

Sonicator Manual<\/a><\/p>\n

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General Notes<\/h3>\n

Sonication disrupts cells (and other biological materials) by transmitting vibrational force into a solution. Microscopic cavities in the solution collapse, \u201ccausing the molecules in the medium to become intensely agitated.\u201d This also causes the solution to heat up so use cool downs between uses and ice to prevent damaging your target material.<\/p>\n

We have a \u201cmicro tip\u201d horn (the horn is the metal part you stick into the solution). Because of this, \u201cdo not advance the Amplitude beyond 40%, or permanent damage to the micro tips can result.\u201d<\/p>\n

Because heat control is so difficult, you may wish to use aluminum (best) or stainless steel containers because the heat conductivity is much better than plastic. Plastic is such a poor heat conductor that should be avoided, or at least use pulsed operation.<\/p>\n

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Usage<\/h3>\n

The power setting, length of time, and number of times has to be individually determined experimentally for each biological material disrupted. Generally, you will want to use low power and short bursts to control heat.<\/p>\n

1) Press the ON key.<\/p>\n

2) Immerse the probe tip into the sample liquid.<\/p>\n

3) To energize the ultrasonics, press the START key. (To de-energize the ultrasonics, press the STOP key)<\/p>\n

4) To set the amplitude when the ultrasonics is off, press the AMP Lkey and
\nthe numeric keys or the \u25b2 or \u25bc key for a % reading on the screen, and then press the ENTER\/REVIEW key.<\/p>\n

5) If you wish for a specific time, Press the TIMER key.<\/p>\n