{"id":134,"date":"2018-05-24T15:28:22","date_gmt":"2018-05-24T15:28:22","guid":{"rendered":"https:\/\/lab.dev.vanderbilt.edu\/cdb-core-lab\/cfx96-qpcr-and-qpcr-touch\/"},"modified":"2021-03-09T17:11:08","modified_gmt":"2021-03-09T17:11:08","slug":"cfx96-qpcr-and-qpcr-touch","status":"publish","type":"page","link":"https:\/\/lab.dev.vanderbilt.edu\/cdb-core-lab\/cfx96-qpcr-and-qpcr-touch\/","title":{"rendered":"CFX96 qPCR and qPCR-TOUCH"},"content":{"rendered":"

Location: U3202 MRB3<\/strong><\/h2>\n

Reservations required in\u00a0 VU iLab website <\/a><\/h3>\n

USERS MUST HAVE THEIR OWN CFX SOFTWARE ACCOUNT ON EACH INSTRUMENT
\n<\/strong><\/p>\n

PLEASE CONTACT THE CORE MANAGER<\/a> IF YOU DO NOT HAVE ONE<\/strong><\/p>\n

The CFX96 Systems \u2014 Designed for the Way You Work<\/h3>\n

The CDB Equipment Resource currently has two CFX96 systems available for use.<\/p>\n

Bio-Rad\u2019s 1000-series PCR instruments meet all your PCR needs \u2014 whether you are designing your first experiment or running a high-throughput laboratory and analyzing large gene expression studies. The CFX96 real-time PCR detection systems build on the power and flexibility of the C1000™ thermal cycler, adding an easy-to-install interchangeable reaction module to create an exceptional real-time PCR system. The system\u2019s unsurpassed thermal performance plus its innovative optical design produce accurate, reliable data, and the powerful, yet intuitive software accelerates every step of your real-time PCR research, shortening the time between getting started and getting great results.<\/p>\n

 <\/p>\n

CFX Manager<\/h3>\n

Want CFX Manager on your computer to analyze data immediately emailed to you when the run is done? Ask me for a copy.<\/strong><\/p>\n

Consumables<\/h3>\n

You will need to purchase your own 96-well plates and plate sealers in addition to your reagents. In the past, the Molecular Biology Core<\/a> has stocked these items. Ordering information is as follows:<\/p>\n

BioRad PCR Plates 96-well clear<\/p>\n

Cat. # MLL9601<\/p>\n

Description: Pkg. of 25, unskirted, low-profile<\/p>\n

 <\/p>\n

BioRad PCR Sealers Microseal “B” Film<\/p>\n

Cat. # MSB1001<\/p>\n

Description: Optically clear, Adhesive seal, Perforation for end-tabs removal, Pack of 100 films<\/p>\n

 <\/p>\n

Background<\/h3>\n

Below, you will find a fantastic resource which is a real time PCR guide. This contains a mountain of information related to the background of the procedure, the various methods available, and critical tips to help you along the way. If you want to be a qPCR expert, this is a must read.
\nWith the CFX96 system, you can:<\/strong><\/p>\n