{"id":129,"date":"2018-05-24T15:28:21","date_gmt":"2018-05-24T15:28:21","guid":{"rendered":"https:\/\/lab.dev.vanderbilt.edu\/cdb-core-lab\/nanodrop-1000\/"},"modified":"2018-09-14T21:05:04","modified_gmt":"2018-09-14T21:05:04","slug":"nanodrop-1000","status":"publish","type":"page","link":"https:\/\/lab.dev.vanderbilt.edu\/cdb-core-lab\/nanodrop-1000\/","title":{"rendered":"Nanodrop 1000"},"content":{"rendered":"<h2><strong>Location: U3202 MRB3<\/strong><\/h2>\n<p>The Nanodrop is a UV\/Vis spectrophotomer with a variety of preset applications. It can measure aqueous nucleic acid samples reproducibly with a 1 \u03bcl samples size and other samples with only 2 \u03bcl. The linear range is quite broad &#8211; minimum absorbance of 2 ng\/\u03bcl and maximum of 3700 ng\/\u03bcl for nucleic acid. Contact the equipment manager for training.<\/p>\n<p>Solvent Compatibility<\/p>\n<p>The Nanodrop 1000C manual says the following on page 19-3: The Nanodrop 1000C Spectrophotometer is compatible with most solvents typically used in life science laboratories.\u00a0 These include: methanol, ethanol, n-propanol, isopropanol, butanol, acetone, ether, chloroform, carbon tetrachloride, DMSO, DMF, Acentronitrile, THF, toluene, hexane, benzene, sodium hydroxide, sodium hypochlorite (bleach), dilute HCl, dilute HNO3, dilute acetic acid.<\/p>\n<p>ALL FORMS OF HYDROFLUORIC ACID (HF) ARE INCOMPATIBLE, AS THE FLUORIDE ION WILL DISSOLVE THE QUARTZ FIBER OPTIC CABLE.<\/p>\n<p><strong>Helpful Hints (from Thermo)<\/strong><\/p>\n<ul>\n<li><strong>Ensure samples are homogeneous<\/strong><\/li>\n<\/ul>\n<p>The samples are small, so it is easy to inadvertently get a non-representative sample for quantitation. There is a vortexer in the equipment room, use it to ensure the your sample is truly in solution (especially nucleic acid).<\/p>\n<ul>\n<li><strong>Use the appropriate buffer to make &#8220;Blank&#8221; solution<\/strong><\/li>\n<\/ul>\n<p>It pains me this even has to be said.<\/p>\n<ul>\n<li><strong>Use adequate sample size<\/strong><\/li>\n<\/ul>\n<p>Use a calibrated 2 \u03bcl pipettor for sample loading &#8211; the larger the pipettor the larger the relative error margin. Volatiles and detergents can sometimes cause problems forming a column and a larger sample volume can help.<\/p>\n<ul>\n<li><strong>Use fresh aliquots for each measurement<\/strong><\/li>\n<\/ul>\n<p>Evaporation can cause a concentration error with the small volume.<\/p>\n<p>&nbsp;<\/p>\n<h3>User Manual<\/h3>\n<p><a href=\"\/wp-content\/uploads\/sites\/21\/public_files\/nd-1000-v3.8-users-manual-8%205x11.pdf\">nd-1000-v3.8-users-manual-8 5&#215;11.pdf <\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Location: U3202 MRB3 The Nanodrop is a UV\/Vis spectrophotomer with a variety of preset applications. It can measure aqueous nucleic acid samples reproducibly with a 1 \u03bcl samples size and other samples with only 2 \u03bcl. The linear range is quite broad &#8211; minimum absorbance of 2 ng\/\u03bcl and maximum of 3700 ng\/\u03bcl for nucleic&#8230;<\/p>\n","protected":false},"author":18,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"open","template":"","meta":{"_acf_changed":false,"jetpack_post_was_ever_published":false,"footnotes":""},"tags":[],"class_list":["post-129","page","type-page","status-publish","hentry"],"acf":[],"jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/P9WyuM-25","_links":{"self":[{"href":"https:\/\/lab.dev.vanderbilt.edu\/cdb-core-lab\/wp-json\/wp\/v2\/pages\/129","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lab.dev.vanderbilt.edu\/cdb-core-lab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/lab.dev.vanderbilt.edu\/cdb-core-lab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/lab.dev.vanderbilt.edu\/cdb-core-lab\/wp-json\/wp\/v2\/users\/18"}],"replies":[{"embeddable":true,"href":"https:\/\/lab.dev.vanderbilt.edu\/cdb-core-lab\/wp-json\/wp\/v2\/comments?post=129"}],"version-history":[{"count":2,"href":"https:\/\/lab.dev.vanderbilt.edu\/cdb-core-lab\/wp-json\/wp\/v2\/pages\/129\/revisions"}],"predecessor-version":[{"id":552,"href":"https:\/\/lab.dev.vanderbilt.edu\/cdb-core-lab\/wp-json\/wp\/v2\/pages\/129\/revisions\/552"}],"wp:attachment":[{"href":"https:\/\/lab.dev.vanderbilt.edu\/cdb-core-lab\/wp-json\/wp\/v2\/media?parent=129"}],"wp:term":[{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lab.dev.vanderbilt.edu\/cdb-core-lab\/wp-json\/wp\/v2\/tags?post=129"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}