Deprecated: Optional parameter $limit declared before required parameter $showlink is implicitly treated as a required parameter in /var/www/m_lab.dev.vanderbilt.edu/wp-content/plugins/wp-pubmed-reflist/class.wpPubMedRefList.php on line 27

Deprecated: Optional parameter $subset declared before required parameter $showlink is implicitly treated as a required parameter in /var/www/m_lab.dev.vanderbilt.edu/wp-content/plugins/wp-pubmed-reflist/class.wpPubMedRefList.php on line 27

Notice: Function _load_textdomain_just_in_time was called incorrectly. Translation loading for the sgg domain was triggered too early. This is usually an indicator for some code in the plugin or theme running too early. Translations should be loaded at the init action or later. Please see Debugging in WordPress for more information. (This message was added in version 6.7.0.) in /var/www/m_lab.dev.vanderbilt.edu/wp-includes/functions.php on line 6114
Rolling blackout, a newly identified PIP2-DAG pathway lipase required for Drosophila phototransduction. | Broadie Laboratory | Vanderbilt University Skip to main content

Rolling blackout, a newly identified PIP2-DAG pathway lipase required for Drosophila phototransduction.


AUTHORS

Huang FDFu-De , Matthies HJ Heinrich J G , Speese SD Sean D , Smith MA Mark A , Broadie K Kendal . Nature neuroscience. 2004 10 ; 7(10). 1070-8

ABSTRACT

The rolling blackout (rbo) gene encodes an integral plasma membrane lipase required for Drosophila phototransduction. Photoreceptors are enriched for the RBO protein, and temperature-sensitive rbo mutants show reversible elimination of phototransduction within minutes, demonstrating an acute requirement for the protein. The block is activity dependent, indicating that the action of RBO is use dependent. Conditional rbo mutants show activity-dependent depletion of diacylglycerol and concomitant accumulation of phosphatidylinositol phosphate and phosphatidylinositol 4,5-bisphosphate within minutes of induction, suggesting rapid downregulation of phospholipase C (PLC) activity. The RBO requirement identifies an essential regulatory step in G-protein-coupled, PLC-dependent inositol lipid signaling mediating activation of TRP and TRPL channels during phototransduction.


The rolling blackout (rbo) gene encodes an integral plasma membrane lipase required for Drosophila phototransduction. Photoreceptors are enriched for the RBO protein, and temperature-sensitive rbo mutants show reversible elimination of phototransduction within minutes, demonstrating an acute requirement for the protein. The block is activity dependent, indicating that the action of RBO is use dependent. Conditional rbo mutants show activity-dependent depletion of diacylglycerol and concomitant accumulation of phosphatidylinositol phosphate and phosphatidylinositol 4,5-bisphosphate within minutes of induction, suggesting rapid downregulation of phospholipase C (PLC) activity. The RBO requirement identifies an essential regulatory step in G-protein-coupled, PLC-dependent inositol lipid signaling mediating activation of TRP and TRPL channels during phototransduction.


Tags:

Leave a Response


Warning: Undefined variable $user_ID in /var/www/m_lab.dev.vanderbilt.edu/wp-content/themes/ANCHORDOWN-Vanderbilt/comments.php on line 62