{"id":104,"date":"2018-06-28T12:40:20","date_gmt":"2018-06-28T12:40:20","guid":{"rendered":"https:\/\/lab.dev.vanderbilt.edu\/bootcampproject6\/integration\/"},"modified":"2018-06-28T13:13:20","modified_gmt":"2018-06-28T13:13:20","slug":"integration","status":"publish","type":"page","link":"https:\/\/lab.dev.vanderbilt.edu\/bootcampproject6\/integration\/","title":{"rendered":"Integration"},"content":{"rendered":"<p class=\"rtejustify\">The Verdict:&nbsp;Integrating C. <em>elegans<\/em> as a Model for Valvulogenesis<\/p>\n<p>As powerful of a model organism C. <em>elegans<\/em> are, there is still applicable research that can be conducted using&nbsp;&nbsp;molecular genetic approaches.&nbsp;<\/p>\n<p>1. <strong>Reverse genetic approach<\/strong>: &nbsp;injection of double stranded RNA (dsRNA) into worms leads to specific degradation of the corresponding mRNA, a process termed RNA interference.&nbsp;This can be done to knock down candidate pharynx specification&nbsp;genes, either one at a time or in combination(s).&nbsp;<\/p>\n<p class=\"rtejustify\">2. <strong>Forward genetic approach<\/strong>: Since C. <em>elegans<\/em> embryos are permeable to small molecules, they could be fed&nbsp;an array of drugs, either one at a time or in combination(s),&nbsp;and the researcher can observe what type of pharyngeal defect&nbsp;forms and then identify the gene(s) that contribute(s) to the phenotype.&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>The Verdict:&nbsp;Integrating C. elegans as a Model for Valvulogenesis As powerful of a model organism C. elegans are, there is still applicable research that can be conducted using&nbsp;&nbsp;molecular genetic approaches.&nbsp; 1. Reverse genetic approach: &nbsp;injection of double stranded RNA (dsRNA) into worms leads to specific degradation of the corresponding mRNA, a process termed RNA interference.&nbsp;This&#8230;<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"open","template":"page_onecolumn.php","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"tags":[],"class_list":["post-104","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/lab.dev.vanderbilt.edu\/bootcampproject6\/wp-json\/wp\/v2\/pages\/104","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lab.dev.vanderbilt.edu\/bootcampproject6\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/lab.dev.vanderbilt.edu\/bootcampproject6\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/lab.dev.vanderbilt.edu\/bootcampproject6\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/lab.dev.vanderbilt.edu\/bootcampproject6\/wp-json\/wp\/v2\/comments?post=104"}],"version-history":[{"count":1,"href":"https:\/\/lab.dev.vanderbilt.edu\/bootcampproject6\/wp-json\/wp\/v2\/pages\/104\/revisions"}],"predecessor-version":[{"id":147,"href":"https:\/\/lab.dev.vanderbilt.edu\/bootcampproject6\/wp-json\/wp\/v2\/pages\/104\/revisions\/147"}],"wp:attachment":[{"href":"https:\/\/lab.dev.vanderbilt.edu\/bootcampproject6\/wp-json\/wp\/v2\/media?parent=104"}],"wp:term":[{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lab.dev.vanderbilt.edu\/bootcampproject6\/wp-json\/wp\/v2\/tags?post=104"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}