{"id":226,"date":"2011-08-25T20:20:58","date_gmt":"2011-08-25T20:20:58","guid":{"rendered":"https:\/\/my.vanderbilt.edu\/alissaweaver\/homepage\/"},"modified":"2019-06-19T16:22:44","modified_gmt":"2019-06-19T16:22:44","slug":"homepage","status":"publish","type":"page","link":"https:\/\/lab.dev.vanderbilt.edu\/alissaweaver\/","title":{"rendered":"Weaver Lab Home Page"},"content":{"rendered":"

Research Description<\/span><\/strong>:<\/p>\n

Cancer metastasis–the spread of cancer cells to distant organs–is what kills the majority of cancer patients.\u00a0 In order for cells to metastasize, they must acquire an invasive and motile phenotype, degrading and moving through tissue barriers.\u00a0 In addition, they must be able to survive and grow at distant sites in the body.<\/p>\n

The Weaver laboratory studies all aspects of this process, focusing particularly on how deregulated signaling in cancer cells drives the invasive and metastatic phenotype.\u00a0 Specific areas are:<\/p>\n

1) Induction of invasive protrusion formation by deregulated signaling.<\/span><\/p>\n

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Figure 2. A molecular interaction network model of invadopodia. Each circle (node) represents a molecule and each line (edge) represents a binding interaction. Nodes that are more highly connected to other nodes are colored in red. Connectivity analysis can be used to identify driver hubs and novel regulators.<\/figcaption><\/figure>\n

The \u201cinvasive phenotype\u201d is characterized by the formation of specific actin-based subcellular structures, including lamellipodial leading edge protrusions that drive forward cell movement and invadopodia (Fig 1) that serve as hotspots for secretion of matrix-degrading proteinases and drive proteinase-dependent invasion. \u00a0These subcellular structures depend on dynamic reorganization of the actin cytoskeleton and are driven in poorly understood ways by signaling and vesicular trafficking. \u00a0Our goal is to understand what drives this process in both normal and cancer cells. \u00a0We are using both computational network analyses (Fig 2), statistical analyses of human tumor molecular data, and traditional cell biological approaches to answer this question. \u00a0Relevant recent publications are:<\/p>\n

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Hoshino, D*, Kirkbride, KC*, Costello, K*, Clark, ES*, Sinha, S*, Grega-Larson, N, Tyska, MJ, and Weaver, AM, “Exosome secretion is enhanced by invadopodia and drives invasive behavior”, Cell Reports, in press, 2013.\u00a0PMID:\u00a024290760<\/a><\/p>\n

Hoshino, D., Jourquin, J., Emmons, S.W., Miller, T., Goldgof, M., Costello, K. Tyson, D.R., Brown, b., Lu, Y., Prasad, N.K., Zhang, B., Mills, G.B., Yarbrough, W.G., Quaranta, V., Seiki, M., Weaver, A.M., “Network Analysis of the Focal Adhesion to Invadopodia Transition Identifies a PI3K-PKCa Invasive Signaling Axis”, Science Signaling, 5: 1-14, 2012. PMCID:\u00a0PMC3583194<\/a><\/p>\n

2) Role of vesicular trafficking in tumor progression<\/span><\/p>\n

Secretion of proteinases, extracellular matrix, and other factors is important during cancer and drives such critical tumor processes, such as invasion, blood vessel formation, and recruitment of stromal cells.\u00a0 Our recent data suggest that specialized secretion is an integral component of cell motility and invasive processes.\u00a0 Interestingly, we recently found that invadopodia are specific docking and secretion sites for exosomes.\u00a0 Exosomes are late endosome-derived extracellular vesicles that are now known to promote various aspects of tumor progression, including angiogenesis and creation of pre-metastatic niches.\u00a0 These data suggest that creation of invadopodia not only promotes proteolysis of the extracellular matrix but also facilitates multiple aspects of tumor progression by enhancing secretion of exosomes.\u00a0 We are currently following up on these findings to understand both how these docking sites are created and the impact for various cellular and tumor phenotypes.<\/p>\n

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Figure 3. Model of exosome secretion at invadopodia. Exosome-carrying multivesicular bodies are docked at invadopodia and secrete growth factor and proteinase-containing exosomes that enhance invadopodia stability, activity and lead to additional de novo invadopodia formation.<\/figcaption><\/figure>\n

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Relevant recent publications are:<\/p>\n

Sung BH, Ketova T, Hoshino D, Zjilstra A, and Weaver AM, “Directional cell movement through tissues is controlled by exosome secretion.” Nat Communications. 2015 May 13; 6:7164. \u00a0PMID:\u00a025968605<\/a><\/p>\n

Hoshino, D*, Kirkbride, KC*, Costello, K*, Clark, ES*, Sinha, S*, Grega-Larson, N, Tyska, MJ, and Weaver, AM, “Exosome secretion is enhanced by invadopodia and drives invasive behavior”, Cell Reports, in press, 2013.\u00a0PMID:\u00a024290760<\/a><\/p>\n

Sung, BH, Xiaodong Xu, Irina Kaverina, and Weaver, A.M., \u201cCortactin Controls Cell Motility and Lamellipodial Dynamics by Regulating ECM Secretion\u201d,\u00a0 Current Biology, 21:1-10, 2011.PMCID:\u00a0PMC3175319<\/a><\/p>\n

3) Trafficking of RNAs in colon cancer exosomes<\/p>\n

Exosomes carry not only protein but also RNA cargoes, including miRNAs.\u00a0 Delivery of RNAs in exosomes has the potential to alter gene expression in the tumor microenvironment and even induce transformation; however more characterization needs to be performed to define which RNAs are enriched in exosomes and whether they are functional.\u00a0 A key to understanding this process is identification of RNA scaffolding and trafficking routes in the cell, as how they are regulated by cancer cell signaling.\u00a0 Our project focuses on the cell biology that underpins the deregulation of this process in colon cancer.\u00a0 This project is part of a national consortium program focused on understanding multiple aspects of extracellular RNA.<\/p>\n

Relevant publications are:<\/p>\n

Mckenzie AJ, Hoshino D, Hong NH, Cha DJ, Frankling JL, Coffey RJ, Patton JG, Weaver AM. “KRAS-MEK Signaling Controls Ago2 Sorting into Exosomes.”\u00a0Cell Reports, S2211-1247(16)30387-4, 2016. PMID: 27117408.
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Research Description: Cancer metastasis–the spread of cancer cells to distant organs–is what kills the majority of cancer patients.\u00a0 In order for cells to metastasize, they must acquire an invasive and motile phenotype, degrading and moving through tissue barriers.\u00a0 In addition, they must be able to survive and grow at distant sites in the body. The…<\/p>\n","protected":false},"author":170,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page_threecolumn.php","meta":{"_acf_changed":false,"footnotes":""},"tags":[],"class_list":["post-226","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/lab.dev.vanderbilt.edu\/alissaweaver\/wp-json\/wp\/v2\/pages\/226","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lab.dev.vanderbilt.edu\/alissaweaver\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/lab.dev.vanderbilt.edu\/alissaweaver\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/lab.dev.vanderbilt.edu\/alissaweaver\/wp-json\/wp\/v2\/users\/170"}],"replies":[{"embeddable":true,"href":"https:\/\/lab.dev.vanderbilt.edu\/alissaweaver\/wp-json\/wp\/v2\/comments?post=226"}],"version-history":[{"count":6,"href":"https:\/\/lab.dev.vanderbilt.edu\/alissaweaver\/wp-json\/wp\/v2\/pages\/226\/revisions"}],"predecessor-version":[{"id":572,"href":"https:\/\/lab.dev.vanderbilt.edu\/alissaweaver\/wp-json\/wp\/v2\/pages\/226\/revisions\/572"}],"wp:attachment":[{"href":"https:\/\/lab.dev.vanderbilt.edu\/alissaweaver\/wp-json\/wp\/v2\/media?parent=226"}],"wp:term":[{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lab.dev.vanderbilt.edu\/alissaweaver\/wp-json\/wp\/v2\/tags?post=226"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}